Journal: Journal of Biomedical Optics
Article Title: Discrimination of lipid composition and cellular localization in human liver tissues by stimulated Raman scattering microscopy
doi: 10.1117/1.JBO.29.1.016008
Figure Lengend Snippet: Schematic diagram of the image analysis pipeline and representative SRS images and lipid composition maps of a liver tissue section from a patient with NASH. From the SRS images at 2850 (a) and 2930 cm − 1 (b), we use the R 2850 / 2930 ratiometric image to create a binary lipid map (c). We then perform the linear least-squares spectral unmixing analysis on both C–H and fingerprint region hyperspectral SRS images of lipid molecules (d) and (e) to generate mole percentage maps of free cholesterol, saturated CE, unsaturated CE, and TAG (f)–(i). The percentage maps of free cholesterol and saturated CE are also shown with a scale extending only from 0% to 10% (for cholesterol) and 0% to 40% (for saturated CE) rather than for 0% to 100% to make more apparent the distribution of these lipids that are present in much lower relative percentages than unsaturated CEs and TAGs. Scale bar: 50 μ m .
Article Snippet: A nonnegative linear least-squares spectral unmixing algorithm (MATLAB Isqnonneg) was applied to both the C–H and the fingerprint region spectra for spectral unmixing of each pixel in lipid droplets.
Techniques: